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Xanthine oxidase traits

1 the appearance of a pale yellow liquid, based crystals suspended in 2.3 mol / L ammonium sulfate, l0mmol / L sodium phosphate buffer solution, containing 1mmol / L EDTA, 1mmol / L sodium salicylate, pH value of approximately 7.8, is iron - molybdenum flavoprotein.
2 can be oxidized hypoxanthine, xanthine, and aldehydes, and the like; the optimum pH value of 8.2, an isoelectric point of 5.3 to 5.4.
3. Activator is oxygen; inhibitors metal ions, cyanides, aldehydes, allopurinol, phosphates, imidazole, sodium chloride and potassium chloride.
4. Crude enzyme can be stored at 2 ℃ weeks vitality without reducing; freezing conditions can be stored for a long.